Coordination of Rho family GTPase activities to orchestrate cytoskeleton responses during cell wound repair.

ABSTRACT:

Background

Cells heal disruptions in their plasma membrane using a sophisticated, efficient, and conserved response involving the formation of a membrane plug and assembly of an actomyosin ring. Here we describe how Rho family GTPases modulate the cytoskeleton machinery during single cell wound repair in the genetically amenable Drosophila embryo model.

Results

We find that Rho, Rac, and Cdc42 rapidly accumulate around the wound and segregate into dynamic, partially overlapping zones. Genetic and pharmacological assays show that each GTPase makes specific contributions to the repair process. Rho1 is necessary for myosin II activation, leading to its association with actin. Rho1, along with Cdc42, is necessary for actin filament formation and subsequent actomyosin ring stabil

SUBMITTER: Abreu-Blanco MT 

PROVIDER: S-EPMC3925435 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature