A9C sensitive Cl⁻-accumulation in A. thaliana root cells during salt stress is controlled by internal and external calcium.

ABSTRACT: The involvement of chloride in salt stress symptoms and salt tolerance mechanisms in plants has been less investigated in the past. Therefore, we studied the salt-induced chloride influx in Arabidopsis expressing the GFP-based anion indicator Clomeleon. High salt concentrations induce two phases of chloride influx. The fast kinetic phase is likely caused by membrane depolarization, and is assumed to be mediated by channels. This is followed by a slower "saturation" phase, where chloride is accumulated in the cytoplasm. Both phases of chloride uptake are dependent on the presence of external calcium. In general: with high [Ca (2+)] less chloride is accumulated in the cytoplasm. Surprisingly, also the internal calcium availability has an impact on chloride transport. A complete block of the

SUBMITTER: Saleh L 

PROVIDER: S-EPMC3925454 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature