Gene expression profiling in subcutaneous, visceral and epigastric adipose tissues of patients with extreme obesity.

ABSTRACT:

Objective

The goal of the present study was to identify differences in gene expression between SAT, VAT and EAT depots in Class III severely obese individuals.

Design

Human subcutaneous (SAT) and visceral (VAT) adipose tissues exhibit differential gene expression profiles. There is little information, however, about the other proximal white adipose tissue, epigastric (EAT), in terms of its function and contribution to metabolism.

Subjects and methods

Using RNA from adipose biospecimens obtained from Class III severely obese patients undergoing open Roux-en-Y gastric bypass surgery, we compared gene expression profiles between SAT, VAT and EAT, using microarrays validated by real-time quantitative PCR.

Results

The three depots were found to share 1907 genes. VAT

SUBMITTER: Gerhard GS 

PROVIDER: S-EPMC3925764 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature