MiR-133b is frequently decreased in gastric cancer and its overexpression reduces the metastatic potential of gastric cancer cells.

ABSTRACT:

Background

Emerging evidence has shown that microRNAs are involved in gastric cancer development and progression. Here we examine the role of miR-133b in gastric cancer.

Methods

Quantitative real-time PCR analysis was performed in 140 patient gastric cancer tissues and 8 gastric cancer cell lines. The effects of miR-133b in gastric cancer cells metastasis were examined by scratch assay, transwell migration and matrigel invasion. In vivo effects of miR-133b were examined in an intraperitoneal mouse tumor model. Targets of miR-133b were predicted by bioinformatics tools and validated by luciferase reporter analyses, western blot, and quantitative real-time PCR.

Results

MiR-133b was significantly downregulated in 70% (98/140) of gastric cancer patients. Expression of miR

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC3925791 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature