Specific gene-regulation networks during the pre-implantation development of the pig embryo as revealed by deep sequencing.

ABSTRACT:

Background

Because few studies exist to describe the unique molecular network regulation behind pig pre-implantation embryonic development (PED), genetic engineering in the pig embryo is limited. Also, this lack of research has hindered derivation and application of porcine embryonic stem cells and porcine induced pluripotent stem cells (iPSCs).

Results

We identified and analyzed the genome wide transcriptomes of pig in vivo-derived and somatic cell nuclear transferred (SCNT) as well as mouse in vivo-derived pre-implantation embryos at different stages using mRNA deep sequencing. Comparison of the pig embryonic transcriptomes with those of mouse and human pre-implantation embryos revealed unique gene expression patterns during pig PED. Pig zygotic genome activation was confi

SUBMITTER: Cao S 

PROVIDER: S-EPMC3925986 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature