DNA-binding specificities of plant transcription factors and their potential to define target genes.

ABSTRACT: Transcription factors (TFs) regulate gene expression through binding to cis-regulatory specific sequences in the promoters of their target genes. In contrast to the genetic code, the transcriptional regulatory code is far from being deciphered and is determined by sequence specificity of TFs, combinatorial cooperation between TFs and chromatin competence. Here we addressed one of these determinants by characterizing the target sequence specificity of 63 plant TFs representing 25 families, using protein-binding microarrays. Remarkably, almost half of these TFs recognized secondary motifs, which in some cases were completely unrelated to the primary element. Analyses of coregulated genes and transcriptomic data from TFs mutants showed the functional significance of over 80% of all identified

SUBMITTER: Franco-Zorrilla JM 

PROVIDER: S-EPMC3926073 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature