RNA sequencing read depth requirement for optimal transcriptome coverage in Hevea brasiliensis.

ABSTRACT:

Background

One of the concerns of assembling de novo transcriptomes is determining the amount of read sequences required to ensure a comprehensive coverage of genes expressed in a particular sample. In this report, we describe the use of Illumina paired-end RNA-Seq (PE RNA-Seq) reads from Hevea brasiliensis (rubber tree) bark to devise a transcript mapping approach for the estimation of the read amount needed for deep transcriptome coverage.

Findings

We optimized the assembly of a Hevea bark transcriptome based on 16 Gb Illumina PE RNA-Seq reads using the Oases assembler across a range of k-mer sizes. We then assessed assembly quality based on transcript N50 length and transcript mapping statistics in relation to (a) known Hevea cDNAs with complete open reading frames, (b) a

SUBMITTER: Chow KS 

PROVIDER: S-EPMC3926681 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature