Chemoenzymatic bio-orthogonal chemistry for site-specific double modification of recombinant thrombomodulin.

ABSTRACT: Best of both worlds: A one-pot strategy for site-specific PEGylation through strain-promoted alkyne-azide cycloaddition (SPAAC) and fluorescent labeling through sortase A-mediated ligation (SML) of recombinant thrombomodulin without prior chemical modification and without diminishing the protein activity has been developed.

SUBMITTER: Jiang R 

PROVIDER: S-EPMC3926800 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature