Application of SNP microarrays to the genome-wide analysis of chromosomal instability in premalignant airway lesions.

ABSTRACT: Chromosomal instability is central to the process of carcinogenesis. The genome-wide detection of somatic chromosomal alterations (SCA) in small premalignant lesions remains challenging because sample heterogeneity dilutes the aberrant cell information. To overcome this hurdle, we focused on the B allele frequency data from single-nucleotide polymorphism microarrays (SNP arrays). The difference of allelic fractions between paired tumor and normal samples from the same patient (delta-θ) provides a simple but sensitive detection of SCA in the affected tissue. We applied the delta-θ approach to small, heterogeneous clinical specimens, including endobronchial biopsies and brushings. Regions identified by delta-θ were validated by FISH and quantitative PCR in heterogeneous samples. Distinctive

SUBMITTER: Nakachi I 

PROVIDER: S-EPMC3926809 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature