DNA-polymer conjugates for immune stimulation through Toll-like receptor 9 mediated pathways.

ABSTRACT: Oligodeoxynucleotides (ODNs) containing unmethylated CpG dinucleotide motifs are agonists of Toll-like receptor 9 and are currently being investigated for use as vaccine adjuvants through the promotion of type I immunity. Several classes of ODN have been developed which differ in their propensity to aggregate, which in turn alters cytokine profiles and cellular subsets activated. Although aggregation state is correlated with the change in cytokine response, it is unknown if this results from a change in the number of ODNs available for binding and/or the possible engagement of multiple TLR9 molecules. Here, we examined the role of ligand valency on the activation of TLR9 through the synthesis of ODN-poly(acrylic acid) (PAA) conjugates. The compositions and size of the conjugates were chara

SUBMITTER: Levenson EA 

PROVIDER: S-EPMC3927139 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature