Tumor-reactive CD8+ T cells in metastatic gastrointestinal cancer refractory to chemotherapy.

ABSTRACT:

Purpose

To evaluate whether patients with metastatic gastrointestinal adenocarcinomas refractory to chemotherapy harbor tumor-reactive cytotoxic T cells.

Experimental design

Expansion of CD8(+) tumor-infiltrating lymphocytes (TIL) and cancer cell lines was attempted from gastrointestinal cancer metastases in 16 consecutive patients for the study of antitumor immune recognition. Retroviral transduction of genes encoding T-cell receptors (TCR) was used to define HLA-restriction elements and specific reactivity.

Results

TIL were expanded from metastases in all patients, and new tumor cell lines were generated in 5 patients. Autologous tumor recognition without cross-reactivity against allogeneic HLA-matched gastrointestinal tumors was found in CD8(+) TIL from 3 of these

SUBMITTER: Turcotte S 

PROVIDER: S-EPMC3927404 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature