Reassessing domain architecture evolution of metazoan proteins: major impact of gene prediction errors.

ABSTRACT: In view of the fact that appearance of novel protein domain architectures (DA) is closely associated with biological innovations, there is a growing interest in the genome-scale reconstruction of the evolutionary history of the domain architectures of multidomain proteins. In such analyses, however, it is usually ignored that a significant proportion of Metazoan sequences analyzed is mispredicted and that this may seriously affect the validity of the conclusions. To estimate the contribution of errors in gene prediction to differences in DA of predicted proteins, we have used the high quality manually curated UniProtKB/Swiss-Prot database as a reference. For genome-scale analysis of domain architectures of predicted proteins we focused on RefSeq, EnsEMBL and NCBI's GNOMON predicted sequenc

SUBMITTER: Nagy A 

PROVIDER: S-EPMC3927609 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature