Burkholderia cenocepacia J2315 escapes to the cytosol and actively subverts autophagy in human macrophages.

ABSTRACT: Selective autophagy functions to specifically degrade cellular cargo tagged by ubiquitination, including bacteria. Strains of the Burkholderia cepacia complex (Bcc) are opportunistic pathogens that cause life-threatening infections in patients with cystic fibrosis (CF) and chronic granulomatous disease (CGD). While there is evidence that defective macrophage autophagy in a mouse model of CF can influence B. cenocepacia susceptibility, there have been no comprehensive studies on how this bacterium is sensed and targeted by the host autophagy response in human macrophages. Here, we describe the intracellular life cycle of B. cenocepacia J2315 and its interaction with the autophagy pathway in human cells. Electron and confocal microscopy analyses demonstrate that the invading bacteria interac

SUBMITTER: Al-Khodor S 

PROVIDER: S-EPMC3927784 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature