Glucose induces rapid changes in the secretome of Saccharomyces cerevisiae.

ABSTRACT:

Background

Protein secretion is a fundamental process in all living cells. Proteins can either be secreted via the classical or non-classical pathways. In Saccharomyces cerevisiae, gluconeogenic enzymes are in the extracellular fraction/periplasm when cells are grown in media containing low glucose. Following a transfer of cells to high glucose media, their levels in the extracellular fraction are reduced rapidly. We hypothesized that changes in the secretome were not restricted to gluconeogenic enzymes. The goal of the current study was to use a proteomic approach to identify extracellular proteins whose levels changed when cells were transferred from low to high glucose media.

Results

We performed two iTRAQ experiments and identified 347 proteins that were present in the e

SUBMITTER: Giardina BJ 

PROVIDER: S-EPMC3927832 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature