Analysis and Modeling of Chromosome Congression During Mitosis in the Chemotherapy Drug Cisplatin.

ABSTRACT: The chemotherapy drug Cisplatin (cis-diamminedichloroplatinum(II)) induces crosslinks within and between DNA strands, and between DNA and nearby proteins. Therefore, Cisplatin-treated cells which progress into cell division may do so with altered chromosome mechanical properties. This could have important consequences for the successful completion of mitosis. Using Total Internal Reflection Fluorescence (TIRF) microscopy of live Cisplatin-treated Saccharomyces cerevisiae cells, we found that metaphase mitotic spindles have disorganized kinetochores relative to untreated cells, and also that there is increased variability in the chromosome stretching distance between sister centromeres. This suggests that chromosome stiffness may become more variable after Cisplatin treatment. We exp

SUBMITTER: Chacon JM 

PROVIDER: S-EPMC3927914 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature