Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells.

ABSTRACT: Human cancers modeled in Genetically Engineered Mouse Models (GEMMs) can provide important mechanistic insights into the molecular basis of tumor development and enable testing of new intervention strategies. The inherent complexity of these models, with often multiple modified tumor suppressor genes and oncogenes, has hampered their use as preclinical models for validating cancer genes and drug targets. In our newly developed approach for the fast generation of tumor cohorts we have overcome this obstacle, as exemplified for three GEMMs; two lung cancer models and one mesothelioma model. Three elements are central for this system; (i) The efficient derivation of authentic Embryonic Stem Cells (ESCs) from established GEMMs, (ii) the routine introduction of transgenes of choice in these GEM

SUBMITTER: Huijbers IJ 

PROVIDER: S-EPMC3927956 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature