Characterization of a fluorescent probe for imaging nitric oxide.

ABSTRACT:

Background

Nitric oxide (NO), a potent vasodilator and anti-atherogenic molecule, is synthesized in various cell types, including vascular endothelial cells (ECs). The biological importance of NO enforces the need to develop and characterize specific and sensitive probes. To date, several fluorophores, chromophores and colorimetric techniques have been developed to detect NO or its metabolites (NO(2) and NO(3)) in biological fluids, viable cells or cell lysates.

Methods

Recently, a novel probe (NO(550)) has been developed and reported to detect NO in solutions and in primary astrocytes and neuronal cells with a fluorescence signal arising from a nonfluorescent background.

Results

Here, we report further characterization of this probe by optimizing conditions for the d

SUBMITTER: Ghebremariam YT 

PROVIDER: S-EPMC3927988 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature