Cerulein-induced chronic pancreatitis does not require intra-acinar activation of trypsinogen in mice.

ABSTRACT:

Background & aims

Premature activation of trypsinogen activation can cause pancreatic injury and has been associated with chronic pancreatitis (CP). Mice that lack intra-acinar activation of trypsinogen, such as trypsinogen-7-null (T(-/-)) and cathepsin B-null (CB(-/-)) mice, have been used to study trypsin-independent processes of CP development. We compared histologic features and inflammatory responses of pancreatic tissues from these mice with those from wild-type mice after the development of CP.

Methods

CP was induced in wild-type, T(-/-), and CB(-/-) mice by twice-weekly induction of acute pancreatitis for 10 weeks; acute pancreatitis was induced by hourly intraperitoneal injections of cerulein (50 μg/kg × 6). Pancreatic samples were collected and evaluated by histolo

SUBMITTER: Sah RP 

PROVIDER: S-EPMC3928043 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature