To nick or not to nick: comparison of I-SceI single- and double-strand break-induced recombination in yeast and human cells.

ABSTRACT: Genetic modification of a chromosomal locus to replace an existing dysfunctional allele with a corrected sequence can be accomplished through targeted gene correction using the cell's homologous recombination (HR) machinery. Gene targeting is stimulated by generation of a DNA double-strand break (DSB) at or near the site of correction, but repair of the break via non-homologous end-joining without using the homologous template can lead to deleterious genomic changes such as in/del mutations, or chromosomal rearrangements. By contrast, generation of a DNA single-strand break (SSB), or nick, can stimulate gene correction without the problems of DSB repair because the uncut DNA strand acts as a template to permit healing without alteration of genetic material. Here, we examine the ability of

SUBMITTER: Katz SS 

PROVIDER: S-EPMC3928301 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature