Highly sensitive quantitative imaging for monitoring single cancer cell growth kinetics and drug response.

ABSTRACT: The detection and treatment of cancer has advanced significantly in the past several decades, with important improvements in our understanding of the fundamental molecular and genetic basis of the disease. Despite these advancements, drug-screening methodologies have remained essentially unchanged since the introduction of the in vitro human cell line screen in 1990. Although the existing methods provide information on the overall effects of compounds on cell viability, they are restricted by bulk measurements, large sample sizes, and lack capability to measure proliferation kinetics at the individual cell level. To truly understand the nature of cancer cell proliferation and to develop personalized adjuvant therapies, there is a need for new methodologies that provide quantitative informa

SUBMITTER: Mir M 

PROVIDER: S-EPMC3928317 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature