Direct imaging of phase objects enables conventional deconvolution in bright field light microscopy.

ABSTRACT: In transmitted optical microscopy, absorption structure and phase structure of the specimen determine the three-dimensional intensity distribution of the image. The elementary impulse responses of the bright field microscope therefore consist of separate absorptive and phase components, precluding general application of linear, conventional deconvolution processing methods to improve image contrast and resolution. However, conventional deconvolution can be applied in the case of pure phase (or pure absorptive) objects if the corresponding phase (or absorptive) impulse responses of the microscope are known. In this work, we present direct measurements of the phase point- and line-spread functions of a high-aperture microscope operating in transmitted bright field. Polystyrene nanoparticles

SUBMITTER: Hernandez Candia CN 

PROVIDER: S-EPMC3928359 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature