Regulation of transcriptional bursting by a naturally oscillating signal.

ABSTRACT: Transcription is highly stochastic, occurring in irregular bursts. For temporal and spatial precision of gene expression, cells must somehow deal with this noisy behavior. To address how this is achieved, we investigated how transcriptional bursting is entrained by a naturally oscillating signal, by direct measurement of transcription together with signal dynamics in living cells. We identify a Dictyostelium gene showing rapid transcriptional oscillations with the same period as extracellular cAMP signaling waves. Bursting approaches antiphase to cAMP waves, with accelerating transcription cycles during differentiation. Although coupling between signal and transcription oscillations was clear at the population level, single-cell transcriptional bursts retained considerable heterogeneity, i

SUBMITTER: Corrigan AM 

PROVIDER: S-EPMC3928820 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature