Retrotaxis of human neutrophils during mechanical confinement inside microfluidic channels.

ABSTRACT: The current paradigm of unidirectional migration of neutrophils from circulation to sites of injury in tissues has been recently challenged by observations in zebrafish showing that neutrophils can return from tissues back into the circulation. However, the relevance of these observations to human neutrophils remains unclear, the forward and reverse migration of neutrophils is difficult to quantify, and the precise conditions modulating the reverse migration cannot be isolated. Here, we designed a microfluidic platform inside which we observed human neutrophil migration in response to chemoattractant sources inside channels, simulating the biochemical and mechanical confinement conditions at sites of injury in tissues. We observed that, after initially following the direction of chemoattra

SUBMITTER: Hamza B 

PROVIDER: S-EPMC3928968 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature