Synthetic LPETG-containing peptide incorporation in the Staphylococcus aureus cell-wall in a sortase A- and growth phase-dependent manner.

ABSTRACT: The majority of Staphylococcus aureus virulence- and colonization-associated surface proteins contain a pentapeptide recognition motif (LPXTG). This motif can be recognized and cleaved by sortase A (SrtA) which is a membrane-bound transpeptidase. After cleavage these proteins are covalently incorporated into the peptidoglycan. Therefore, SrtA plays a key role in S. aureus virulence. We aimed to generate a substrate mimicking this SrtA recognition motif for several purposes: to incorporate this substrate into the S. aureus cell-wall in a SrtA-dependent manner, to characterize this incorporation and to determine the effect of substrate incorporation on the incorporation of native SrtA-dependent cell-surface-associated proteins. We synthesized substrate containing the specific LPXTG motif, LP

SUBMITTER: Hansenova Manaskova S 

PROVIDER: S-EPMC3929722 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature