Purification, characterization and production optimization of a vibriocin produced by mangrove associated Vibrio parahaemolyticus.

ABSTRACT:

Objective

To identify a potential bacterium which produces antimicrobial peptide (vibriocin), and its purification, characterization and production optimization. The bacteria subjected in the study were isolated from a highly competitive ecological niche of mangrove ecosystem.

Methods

The bacterium was characterized by phenotype besides 16S rRNA gene sequence analysis. The antibacterial activity was recognised by using agar well diffusion method. The vibriocin was purified using ammonium sulphate precipitation, butanol extraction, gel filtration chromatography, ion-exchange chromatography and subsequently, by HPLC. Molecular weight of the substance identified in SDS-PAGE. Production optimization performed according to Taguchi's mathematical model using 6 different nutritiona

SUBMITTER: Balakrishnan B 

PROVIDER: S-EPMC3929786 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature