Circulating miR-378 in plasma: a reliable, haemolysis-independent biomarker for colorectal cancer.

ABSTRACT:

Background

Plasma circulating tumour-specific microRNAs (miRNAs) are promising biomarkers of tumour presence and recurrence, especially for diseases whose best chance of successful treatment requires early diagnosis and timely surgery of an already malignant but not yet invasive tumour, such as colorectal cancer (CRC).

Methods

Expression levels of miRNAs previously found to be differently expressed in tumour vs normal colon tissues were investigated by quantitative real-time PCR in plasma from CRC patients and from healthy donors and confirmed in independent case control series. The validated miRNAs were also measured after surgery. Analyses were repeated on the subsets of haemolysis-free samples.

Results

We identified four miRNAs differently expressed between the com

SUBMITTER: Zanutto S 

PROVIDER: S-EPMC3929896 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature