Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo.

ABSTRACT: We describe here the development and characterization of a conditionally inducible mouse model expressing Lifeact-GFP, a peptide that reports the dynamics of filamentous actin. We have used this model to study platelets, megakaryocytes and melanoblasts and we provide evidence that Lifeact-GFP is a useful reporter in these cell types ex vivo. In the case of platelets and megakaryocytes, these cells are not transfectable by traditional methods, so conditional activation of Lifeact allows the study of actin dynamics in these cells live. We studied melanoblasts in native skin explants from embryos, allowing the visualization of live actin dynamics during cytokinesis and migration. Our study revealed that melanoblasts lacking the small GTPase Rac1 show a delay in the formation of new pseudopodi

SUBMITTER: Schachtner H 

PROVIDER: S-EPMC3930012 | biostudies-literature | 2012 Nov-Dec

REPOSITORIES: biostudies-literature