Genetic and functional properties of uncultivated MCG archaea assessed by metagenome and gene expression analyses.

ABSTRACT: The Miscellaneous Crenarchaeota group (MCG) Archaea is one of the predominant archaeal groups in anoxic environments and may have significant roles in the global biogeochemical cycles. However, no isolate of MCG has been cultivated or characterized to date. In this study, we investigated the genetic organization, ecophysiological properties and evolutionary relationships of MCG archaea with other archaeal members using metagenome information and the result of gene expression experiments. A comparison of the gene organizations and similarities around the 16S rRNA genes from all available MCG fosmid and cosmid clones revealed no significant synteny among genomic fragments, demonstrating that there are large genetic variations within members of the MCG. Phylogenetic analyses of large-subunit+

SUBMITTER: Meng J 

PROVIDER: S-EPMC3930316 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature