Systematic quantification of developmental phenotypes at single-cell resolution during embryogenesis.

ABSTRACT: Current imaging technology provides an experimental platform in which complex developmental processes can be observed at cellular resolution over an extended time frame. New computational tools are essential to achieve a systems-level understanding of this high-content information. We have devised a structured approach to systematically analyze complex in vivo phenotypes at cellular resolution, which divides the task into a panel of statistical measurements of each cell in terms of cell differentiation, proliferation and morphogenesis, followed by their spatial and temporal organization in groups and the cohesion within the whole specimen. We demonstrate the approach to C. elegans embryogenesis with in toto imaging and automated cell lineage tracing. We define statistical distributions of

SUBMITTER: Moore JL 

PROVIDER: S-EPMC3931741 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature