Systematical identification of splicing regulatory cis-elements and cognate trans-factors.

ABSTRACT: The majority of human genes undergo alternative splicing to generate multiple isoforms with distinct functions. This process is generally controlled by cis-acting splicing regulatory elements (SREs) that recruit trans-acting factors to promote or inhibit the use of nearby splice sites. The growing interest in understanding the regulatory rules of splicing necessitates the systematic identification of these SREs and their cognate protein factors using experimental and computational approaches. Here we describe a strategy to identify and analyze both cis-acting SREs and trans-acting splicing factors. This strategy involves a cell-based screen to identify SREs from a random sequences library and a modified RNA affinity purification approach to unbiasedly identify the splicing factors. These m

SUBMITTER: Wang Y 

PROVIDER: S-EPMC3932149 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature