Chlamydia trachomatis-induced alterations in the host cell proteome are required for intracellular growth.

ABSTRACT: Intracellular pathogens directly alter host cells in order to replicate and survive. While infection-induced changes in host transcription can be readily assessed, posttranscriptional alterations are more difficult to catalog. We applied the global protein stability (GPS) platform, which assesses protein stability based on relative changes in an adjoining fluorescent tag, to identify changes in the host proteome following infection with the obligate intracellular bacteria Chlamydia trachomatis. Our results indicate that C. trachomatis profoundly remodels the host proteome independently of changes in transcription. Additionally, C. trachomatis replication depends on a subset of altered proteins, such as Pin1 and Men1, that regulate the host transcription factor AP-1 controlling host inflamm

SUBMITTER: Olive AJ 

PROVIDER: S-EPMC3932326 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature