Suppression of Mediator is regulated by Cdk8-dependent Grr1 turnover of the Med3 coactivator.

ABSTRACT: Mediator, an evolutionary conserved large multisubunit protein complex with a central role in regulating RNA polymerase II-transcribed genes, serves as a molecular switchboard at the interface between DNA binding transcription factors and the general transcription machinery. Mediator subunits include the Cdk8 module, which has both positive and negative effects on activator-dependent transcription through the activity of the cyclin-dependent kinase Cdk8, and the tail module, which is required for positive and negative regulation of transcription, correct preinitiation complex formation in basal and activated transcription, and Mediator recruitment. Currently, the molecular mechanisms governing Mediator function remain largely undefined. Here we demonstrate an autoregulatory mechanism used

SUBMITTER: Gonzalez D 

PROVIDER: S-EPMC3932902 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature