Central role for RNase YbeY in Hfq-dependent and Hfq-independent small-RNA regulation in bacteria.

ABSTRACT:

Background

Conceptual parallels exist between bacterial and eukaryotic small-RNA (sRNA) pathways, yet relatively little is known about which protein may recognize and recruit bacterial sRNAs to interact with targets. In eukaryotes, Argonaute (AGO) proteins discharge such functions. The highly conserved bacterial YbeY RNase has structural similarities to the MID domain of AGOs. A limited study had indicated that in Sinorhizobium meliloti the YbeY ortholog regulates the accumulation of sRNAs as well as the target mRNAs, raising the possibility that YbeY may play a previously unrecognized role in bacterial sRNA regulation.

Results

We have applied a multipronged approach of loss-of-function studies, genome-wide mRNA and sRNA expression profiling, pathway analysis, target predict

SUBMITTER: Pandey SP 

PROVIDER: S-EPMC3933206 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature