RtM204Q may serve as a novel lamivudine-resistance-associated mutation of hepatitis B virus.

ABSTRACT:

Background and aims

Lamivudine (LAM) is still widely used for anti-HBV therapy in China. The study aimed to clarify whether a newly-found rtM204Q mutation from patients was associated with the drug resistance.

Methods

HBV complete reverse-transcriptase region was screened by direct sequencing and verified by clonal sequencing. Replication-competent plasmids containing patient-derived 1.1mer mutant or wild-type viral genome were constructed and transfected into HepG2 cells. After cultured with or without serially-diluted antiviral drugs, intracellular HBV replicative intermediates were quantitated for calculating the 50% effective concentration of drug (EC₅₀).

Results

A total of 12,000 serum samples of 9,830 patients with chronic HBV infection were screened. rtM204Q mu

SUBMITTER: Liu Y 

PROVIDER: S-EPMC3933355 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature