Stauprimide Priming of Human Embryonic Stem Cells toward Definitive Endoderm.

ABSTRACT:

Objective

In vitro production of a definitive endoderm (DE) is an important issue in stem cell-related differentiation studies and it can assist with the production of more efficient endoderm derivatives for therapeutic applications. Despite tremendous progress in DE differentiation of human embryonic stem cells (hESCs), researchers have yet to discover universal, efficient and cost-effective protocols.

Materials and methods

In this experimental study, we have treated hESCs with 200 nM of Stauprimide (Spd) for one day followed by activin A (50 ng/ml; A50) for the next three days (Spd-A50). In the positive control group, hESCs were treated with Wnt3a (25 ng/ml) and activin A (100 ng/ml) for the first day followed by activin A for the next three days (100 ng/ml; W/A100-A100).<

SUBMITTER: Tahamtani Y 

PROVIDER: S-EPMC3933440 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature