Targeted metabolomics identifies reliable and stable metabolites in human serum and plasma samples.

ABSTRACT:

Background

Information regarding the variability of metabolite levels over time in an individual is required to estimate the reproducibility of metabolite measurements. In intervention studies, it is critical to appropriately judge changes that are elicited by any kind of intervention. The pre-analytic phase (collection, transport and sample processing) is a particularly important component of data quality in multi-center studies.

Methods

Reliability of metabolites (within-and between-person variance, intraclass correlation coefficient) and stability (shipment simulation at different temperatures, use of gel-barrier collection tubes, freeze-thaw cycles) were analyzed in fasting serum and plasma samples of 22 healthy human subjects using a targeted LC-MS approach.

Results<

SUBMITTER: Breier M 

PROVIDER: S-EPMC3933650 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature