Hypoxia exerts dualistic effects on inflammatory and proliferative responses of healthy and asthmatic primary human bronchial smooth muscle cells.

ABSTRACT:

Background

For oxygen supply, airway wall cells depend on diffusion though the basement membrane, as well as on delivery by micro-vessels. In the asthmatic lung, local hypoxic conditions may occur due to increased thickness and altered composition of the basement membrane, as well as due to edema of the inflamed airway wall.

Objective

In our study we investigated the effect of hypoxia on proliferation and pro-inflammatory and pro-angiogenic parameter production by human bronchial smooth muscle cells (BSMC). Furthermore, conditioned media of hypoxia-exposed BSMC was tested for its ability to induce sprout outgrowth from endothelial cells spheroids.

Methods

BSMC were cultured in RPMI1640 (5% FCS) under normoxic (21% O₂) and hypoxic (1% and 5% O₂) conditions. Proliferati

SUBMITTER: Keglowich L 

PROVIDER: S-EPMC3933675 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature