Structure and biomechanics of the endothelial transcellular circumferential invasion array in tumor invasion.

ABSTRACT: Cancer cells breach the endothelium not only through cell-cell junctions but also via individual endothelial cells (ECs), or transcellular invasion. The underlying EC forms a circular structure around the transcellular invasion pore that is dependent on myosin light chain kinase (MLCK) and myosin II regulatory light chain (RLC) phosphorylation. Here we offer mechanistic insights into transcellular invasive array formation amid persistent tensile force from activated EC myosin. Fluorescence recovery after photobleaching (FRAP) experiments, sarcomeric distance measurements using super-resolution microscopy and electron microscopy provide details about the nature of the myosin II invasion array. To probe the relationship between biomechanical forces and the tension required to maintain the cu

SUBMITTER: Arvanitis C 

PROVIDER: S-EPMC3933692 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature