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Stereological and ultrastructural quantification of the afferent synaptome of individual neurons.

ABSTRACT: Determining the number and placement of synaptic inputs along the distinct plasma membrane domains of neurons is essential for explaining the basis of neuronal activity and function. We detail a strategy that combines juxtacellular labeling, neuronal reconstructions and stereological sampling of inputs at the ultrastructural level to define key elements of the afferent 'synaptome' of a given neuron. This approach provides unbiased estimates of the total number and somato-dendritic distribution of synapses made with individual neurons. These organizational properties can be related to the activity of the same neurons previously recorded in vivo, for direct structure-function correlations at the single-cell level. The approach also provides the quantitative data required to develop biologically realistic models that simulate and predict neuronal activity and function.

SUBMITTER: Henny P 

PROVIDER: S-EPMC3933745 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Stereological and ultrastructural quantification of the afferent synaptome of individual neurons.

Henny Pablo P   Brown Matthew T C MT   Micklem Benjamin R BR   Magill Peter J PJ   Bolam J Paul JP  

Brain structure & function 20130312 2

Determining the number and placement of synaptic inputs along the distinct plasma membrane domains of neurons is essential for explaining the basis of neuronal activity and function. We detail a strategy that combines juxtacellular labeling, neuronal reconstructions and stereological sampling of inputs at the ultrastructural level to define key elements of the afferent 'synaptome' of a given neuron. This approach provides unbiased estimates of the total number and somato-dendritic distribution o  ...[more]