Extravascular CX3CR1+ cells extend intravascular dendritic processes into intact central nervous system vessel lumen.

ABSTRACT: Within the central nervous system (CNS), antigen-presenting cells (APCs) play a critical role in orchestrating inflammatory responses where they present CNS-derived antigens to immune cells that are recruited from the circulation to the cerebrospinal fluid, parenchyma, and perivascular space. Available data indicate that APCs do so indirectly from outside of CNS vessels without direct access to luminal contents. Here, we applied high-resolution, dynamic intravital two-photon laser scanning microscopy to directly visualize extravascular CX3CR1+ APC behavior deep within undisrupted CNS tissues in two distinct anatomical sites under three different inflammatory stimuli. Surprisingly, we observed that CNS-resident APCs dynamically extend their cellular processes across an intact vessel wall in

SUBMITTER: Barkauskas DS 

PROVIDER: S-EPMC3933819 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature