Electron paramagnetic resonance resolves effects of oxidative stress on muscle proteins.

ABSTRACT: We have used site-directed spin labeling and electron paramagnetic resonance (EPR) to explore the effects of oxidation on muscle function, with particular focus on the actin-myosin interaction. EPR measurements show that aging or oxidative modification causes a decrease in the fraction of myosins in the strong-binding state, which can be traced to the actin-binding cleft of the myosin catalytic domain.

SUBMITTER: Moen RJ 

PROVIDER: S-EPMC3934098 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature