Ca2+ responses in enteric glia are mediated by connexin-43 hemichannels and modulate colonic transit in mice.

ABSTRACT:

Background & aims

In the enteric nervous system, neurotransmitters initiate changes in calcium (Ca(2+) responses) in glia, but it is not clear how this process affects intestinal function. We investigated whether Ca(2+)-mediated responses in enteric glia are required to maintain gastrointestinal function.

Methods

We used in situ Ca(2+) imaging to monitor glial Ca(2+) responses, which were manipulated with pharmacologic agents or via glia-specific disruption of the gene encoding connexin-43 (Cx43) (hGFAP::CreER(T2+/-)/Cx43(f/f) mice). Gastrointestinal function was assessed based on pellet output, total gut transit, colonic bead expulsion, and muscle tension recordings. Proteins were localized and quantified by immunohistochemistry, immunoblot, and reverse transcription polyme

SUBMITTER: McClain J 

PROVIDER: S-EPMC3935238 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature