Structural analysis of viral infectivity factor of HIV type 1 and its interaction with A3G, EloC and EloB.

ABSTRACT:

Background

The virion infectivity factor (Vif) is an accessory protein, which is essential for HIV replication in host cells. Vif neutralizes the antiviral host protein APOBEC3 through recruitment of the E3 ubiquitin ligase complex.

Methodology

Fifty thousand Vif models were generated using the ab initio relax protocol of the Rosetta algorithm from sets of three- and nine-residue fragments using the fragment Monte Carlo insertion-simulated annealing strategy, which favors protein-like features, followed by an all-atom refinement. In the protocol, a constraints archive was used to define the spatial relationship between the side chains from Cys/His residues and zinc ions that formed the zinc-finger motif that is essential for Vif function. We also performed centroids analysis

SUBMITTER: da Costa KS 

PROVIDER: S-EPMC3935857 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature