Natural cardiogenesis-based template predicts cardiogenic potential of induced pluripotent stem cell lines.

ABSTRACT:

Background

Cardiac development is a complex process resulting in an integrated, multilineage tissue with developmental corruption in early embryogenesis leading to congenital heart disease. Interrogation of individual genes has provided the backbone for cardiac developmental biology, yet a comprehensive transcriptome derived from natural cardiogenesis is required to gauge innate developmental milestones.

Methods and results

Stage-specific cardiac structures were dissected from 8 distinctive mouse embryonic time points to produce genome-wide expressome analysis across cardiogenesis. With reference to this native cardiogenic expression roadmap, divergent induced pluripotent stem cell-derived cardiac expression profiles were mapped from procardiogenic 3-factor (SOX2, OCT4, KLF4

SUBMITTER: Martinez-Fernandez A 

PROVIDER: S-EPMC3936313 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature