Unraveling the redox properties of the global regulator FurA from Anabaena sp. PCC 7120: disulfide reductase activity based on its CXXC motifs.

ABSTRACT:

Unlabelled

Cyanobacterial FurA works as a global regulator linking iron homeostasis to photosynthetic metabolism and the responses to different environmental stresses. Additionally, FurA modulates several genes involved in redox homeostasis and fulfills the characteristics of a heme-sensor protein whose interaction with this cofactor negatively affects its DNA binding ability. FurA from Anabaena PCC 7120 contains five cysteine residues, four of them arranged in two redox CXXC motifs.

Aims

Our goals were to analyze in depth the putative contribution of these CXXC motifs in the redox properties of FurA and to identify potential interacting partners of this regulator.

Results

Insulin reduction assays unravel that FurA exhibits disulfide reductase activity. Simultaneous p

SUBMITTER: Botello-Morte L 

PROVIDER: S-EPMC3936511 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature