Effects of nitrogen nutrition on the synthesis and deposition of the ω-gliadins of wheat.

ABSTRACT:

Background and aims

The ω-gliadin storage proteins of wheat are of interest in relation to their impact on grain processing properties and their role in food allergy, particularly the ω-5 sub-group and wheat-dependent exercise-induced anaphylaxis. The ω-gliadins are also known to be responsive to nitrogen application. This study therefore compares the effects of cultivar and nitrogen availability on the synthesis and deposition of ω-gliadins in wheat grown under field conditions in the UK, including temporal and spatial analyses at the protein and transcript levels.

Methods

SDS-PAGE, western blotting and N-terminal amino acid sequencing were used to compare the patterns of ω-gliadin components in mature grain of six British wheat (Triticum aestivum) cultivars and their accum

SUBMITTER: Wan Y 

PROVIDER: S-EPMC3936585 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature