Folate Levels and Polymorphisms in the Genes MTHFR, MTR, and TS in Colorectal Cancer.

ABSTRACT:

Aim

The aim of the study was to explore and describe the effect of polymorphisms in folate-associated genes regarding the levels of different folate forms and their distribution in tumors and mucosa in patients with colorectal cancer.

Materials and methods

Tumor and mucosa tissues from 53 patients with colorectal cancer were analyzed. The concentrations of tetrahydrofolate (THF), 5-methylTHF, and 5,10-methyleneTHF were measured by liquid chromatography-mass spectrometry. Genotyping of polymorphisms in the folate-associated genes methylenetetrahydrofolate reductase (MTHFR, C677T), methionine synthase (MTR, A2756G), and thymidylate synthase (TS, 5'-TSER 28 bp tandem repeat and 3'-TSUTR 6 bp deletion/insertion), were done by real-time polymerase chain reaction. Folate levels an

SUBMITTER: Taflin H 

PROVIDER: S-EPMC3937179 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature