G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane.

ABSTRACT:

Background

Animal steroid hormones are conventionally known to initiate signaling via a genomic pathway by binding to the nuclear receptors. The mechanism by which 20E initiates signaling via a nongenomic pathway is unclear.

Results

We illustrate that 20E triggered the nongenomic pathway through a plasma membrane G-protein-coupled receptor (named ErGPCR) in the lepidopteran insect Helicoverpa armigera. The transcript of ErGPCR was increased at the larval molting stage and metamorphic molting stage by 20E regulation. Knockdown of ErGPCR via RNA interference in vivo blocked larval-pupal transition and suppressed 20E-induced gene expression. ErGPCR overexpression in the H. armigera epidermal cell line increased the 20E-induced gene expression. Through ErGPCR, 20E modulated Calp

SUBMITTER: Cai MJ 

PROVIDER: S-EPMC3937218 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature