The secreted triose phosphate isomerase of Brugia malayi is required to sustain microfilaria production in vivo.

ABSTRACT: Human lymphatic filariasis is a major tropical disease transmitted through mosquito vectors which take up microfilarial larvae from the blood of infected subjects. Microfilariae are produced by long-lived adult parasites, which also release a suite of excretory-secretory products that have recently been subject to in-depth proteomic analysis. Surprisingly, the most abundant secreted protein of adult Brugia malayi is triose phosphate isomerase (TPI), a glycolytic enzyme usually associated with the cytosol. We now show that while TPI is a prominent target of the antibody response to infection, there is little antibody-mediated inhibition of catalytic activity by polyclonal sera. We generated a panel of twenty-three anti-TPI monoclonal antibodies and found only two were able to block TPI enzy

SUBMITTER: Hewitson JP 

PROVIDER: S-EPMC3937304 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature