Acrolein modification impairs key functional features of rat apolipoprotein E: identification of modified sites by mass spectrometry.

ABSTRACT: Apolipoprotein E (apoE), an antiatherogenic apolipoprotein, plays a significant role in the metabolism of lipoproteins. It lowers plasma lipid levels by acting as a ligand for the low-density lipoprotein receptor (LDLr) family of proteins, in addition to playing a role in promoting macrophage cholesterol efflux in atherosclerotic lesions. The objective of this study is to examine the effect of acrolein modification on the structure and function of rat apoE and to determine the sites and nature of modification by mass spectrometry. Acrolein is a highly reactive aldehyde, which is generated endogenously as one of the products of lipid peroxidation and is present in the environment in pollutants such as tobacco smoke and heated oils. In initial studies, acrolein-modified apoE was identified b

SUBMITTER: Tran TN 

PROVIDER: S-EPMC3937454 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature